![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3279002:Design
Designed by: Shujie Liao Group: iGEM19_CAU_China (2019-10-14)
Phytoene desaturase (CrtI) from Rhodospirillum rubrum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 779
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 766
Illegal NgoMIV site found at 1450 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 325
Illegal BsaI.rc site found at 820
Illegal SapI.rc site found at 232
Design Notes
The primary concern was BioBrick compatibility so the illegal sites in RFC[10] did not appear in the sequence. And the sequence was finally designed to be cloned into pACYC184-M by Gibson assembly.
Source
This part comes from Rhodospirillum rubrum's genomic sequence.