Coding

Part:BBa_K3279002:Design

Designed by: Shujie Liao   Group: iGEM19_CAU_China   (2019-10-14)


Phytoene desaturase (CrtI) from Rhodospirillum rubrum


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 779
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 766
    Illegal NgoMIV site found at 1450
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 325
    Illegal BsaI.rc site found at 820
    Illegal SapI.rc site found at 232


Design Notes

The primary concern was BioBrick compatibility so the illegal sites in RFC[10] did not appear in the sequence. And the sequence was finally designed to be cloned into pACYC184-M by Gibson assembly.


Source

This part comes from Rhodospirillum rubrum's genomic sequence.

References